1. What is your hypothesis regarding the genotype of the sporophyte that produce the spores we used in this experiment?
2. What is your hypothesis regarding the type of dominance controlling the pigmentation gene? This is your genetic hypothesis.
3. What is your Chi-Square hypothesis for the analysis you will do to address each of the first two hypotheses, above? This hypothesis is always the same. (See the Chi-Square Supplement to the C-fern lab)
4. Do you understand how to calculate Chi-Square, degrees of freedom, and use the table of critical values? (See the Chi-Square Supplement to the C-fern lab)
5. Are your Chi-square hypotheses accepted or rejected? How does this affect your evaluation of your other experimental hypotheses? (Remember, hypotheses are never proven – only supported or rejected.)
6. Are the data and your chi-square results what you expected, based upon what you should know about meiosis, Mendelian genetics, and an alternation of generations life cycle (sporic meiosis)? If the data and chi-square analyses are not what you expected, explain what you were expecting (including genetic ratios) and possible sources of problems.
7. What are the genotypes possible in the gametes produced by each of the different phenotypes of gametophyte (solid green = wild type or polka dot = mutant)?