One-step Growth Experiment

The protocol used in this exercise cannot provide a truly rigorous determination of the SP6 burst size, which
would require a modification of the protocol or additional information not supplied by this experiment. Explain
what assumption needs to be made to compute the burst size as described above (Instructions for Day 2).
What is wrong with this assumption? How might you do this experiment (or additional experiments) to more
rigorously determine the SP6 burst size.