Organic Materials and Nutrition

1) True or False. Nutrient Broth and Nutrient Agar are common media used for maintaining bacterial cultures. 2) It is important to transfer living microbes from one place to another aseptically. What does the term aseptically mean? 3) A medium that contains living microbes is called a . If a culture contains a single species it is said to be a . 4) Plated media are typically used for a. nutrient broth preparation b. creating multiple DNA strands c. obtaining isolation of species d. blood typing 5) Draw below an example of a zigzag inoculation and a quadrant streak. 6) What were you selecting for in the pGreen Transformation experiment? (i.e., what allows you to identify which bacteria have taken up the plasmid?) a. Cells that did not contain plasmid DNA b. Resistance to ampicillin c. Temperature of incubation period d. Cells that formed a lawn 7) Which petri dish in the pGreen Transformation experiment should have produced visible colonies? a. LB+plasmid b. LB-plasmid c. LB/Amp+plasmid d. LB/Amp-plasmid 8) Which petri dish in the pGreen Transformation experiment should have produced no colonies? a. LB+plasmid b. LB-plasmid c. LB/Amp+plasmid d. LB/Amp-plasmid 9) Which fingerprint pattern should an exceedingly unusual pattern be classified under if it may not be placed into any other classes? a. Plain Arch b. Plain Whorl c. Accidental Loop d. Accidental Whorl 10) True or False. A negative agglutination reaction indicates the blood type. 11) During blood typing, a blood sample was found to have positive agglutination for red cell antigen A and red cell antigen B. What is the blood type of the sample? a. A b. B c. O d. AB 12) Larvae was found on a dead body and measured to estimate the number of days since death. The measurement of the smallest larvae was 9mm and the measurement of the largest larvae was 16mm. When did this individual die? a. 6 days b. 8 days c. 4 days d. 3 days 13) A wastewater pollutant that is most difficult to remove is a. Nonpolar liquids b. Primary sludge c. Colloidal suspensions d. Secondary sludge 14) True or False. Liquids that are classified as immiscible are those that will not mix with water. 15) At what stage of wastewater treatment does aeration occur? a. Preliminary b. Primary c. Secondary d. Tertiary 16) What does the alum and sodium hydroxide create in the wastewater treatment tank? a. precipitate b. filtration c. turbidity d. suspended solids 17) True or False. Cuvette 1 of the plant photosynthesis experiment is important for setting the spectrophotometer to 1% transmittance. 18) Cuvette 5 was used as the control in your plant photosynthesis experiment. It contained all the test ingredients except chloroplasts. Why would this be considered a control? a. It contained chloroplasts b. It can’t be compared to the other cuvettes because it does not have chloroplasts c. It showed that plants don’t use photosynthesis d. It showed that any change in light transmittance cannot be the result of photosynthesis 19) Cuvettes 2 and 3 demonstrated reactions of photosynthesis. These reactions are • Cuvette 2 demonstrated that • Cuvette 3 demonstrated that 20) Cuvette 4 tests the effect of heat on the ability of the chloroplast suspension to conduct the light reactions in the presence of light. Why did heat affect the outcome? 21) Detecting patterns or changes in transcription in cells is a way to understand what aspects of cell function? a. Normal b. Abnormal c. Gene Chip d. A & B 22) True or False. A single microarray can contain more than 30,000 spots of DNA, each representing a different gene in an organism. 23) Regarding gene expression and cancer, which genes may play a role in causing cancer in cells? Genes expressed in normal cells Genes expressed in cancer cells May play a role in causing cancer? (yes or no) High Low Low High Low Not expressed Not expressed Low Same level Same level Not expressed Not expressed 24) Which is a virus? a. AIDS b. HIV c. ELISA d. Opportunistic Infection 25) Which is NOT an opportunistic infection? a. Pneumonia b. Asthma c. Kaposi’s sarcoma d. Thrush 26) Benedict’s solution tests for . 27) Iodine tests for . 28) Sudan IV tests for . 29) Biuret test is for . 30) Freebie :)