tyrosine phosphorylation in E.coli

  Paper details: Task Write up a publication on the work that we have undertaken in the laboratory component of ART. We will be submitting this paper to the following Journal: Journal of Bacteriology. Note for figures- Please include these as either incorporated in your word file document or as a separate powerpoint files- Word count should be a MAXIMUM of 3000 words- Abstract (max 200 words): The Abstract section should be 200 words or fewer concisely summarizing the basic content of the paper without extensive experimental details- No references should be included in this section- Introduction (800 words)- The introduction should supply sufficient background information to allow the reader to understand and evaluate the results of the present study without referring to previous publications on the topic- The introduction should also provide the hypothesis that was addressed or the rationale for the present study. Use only those references required to provide the most salient background rather than an exhaustive review of the topic- Some tips as to what to include (You can use your answer to question one of Assignment one as the basis for this) ‘I- What is tyrosine phosphorylation? 2- Why is it important? What does it do? Provide at least 2 specific examples- (This can concentrate on bacterial examples for now, but remember that eukaryotes also use tyrosine phosphorylation-) 3- How is it predominantly studied now? What is wrong with the current use of mimics? (Use a Figure if you like) 4- What are we going to do that is different? How? 5- What tyrosine kinase are we using? Why is it a fusion of two proteins? Materials and Methods (500 words)- The Materials and Methods section should include sufficient technical information to allow the experiments to be repeated- When centrifugation conditions are critical, give enough information to enable another investigator to repeat the procedure: make of centrifuge, model of rotor, temperature, time at maximum speed, and centrifugal force (x g rather than revolutions per minute)-