Pour plate and streaking technique to obtain pure cultures

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1. Discuss the relative convenience of pour- and streak-plate techniques in culturing clinical
specimens.
2. How do you decide which colonies should be picked from a plate culture of a mixed flora?
3. Why is it necessary to make pure subcultures of organisms grown from clinical specimens?
4. What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
5. When more than one colony type appears in pure culture, what are the most likely sources of
extraneous contamination?

 

 

 

 

 

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