find the bacterial transformation assessment attached ( Virtual Bacterial Transformation Lab Extension Fall 2022.docx Virtual Bacterial Transformation Lab Extension Fall 2022.docx – Alternative Formats ). Be sure to complete the assessment as indicated in the document and upload the assessment as directed in Blackboard. While the molecular cloning lab simulation should be helpful, you will find additional information regarding resources to help you as you work through the assessment below.
For this lab assessment, you will be applying your knowledge from many areas of the course this semester to explore the impact that a promoter sequence can have on gene expression. Please watch part 1 of the first video and review the second video as well as a way of familiarizing yourself with the basics of a bacterial transformation procedure.
Part 1 only: https://www.youtube.com/watch?v=OZyFX9megs8
A summary: https://www.youtube.com/watch?v=slY4qrnZIM8
Below, please find a brief overview of the assignment and where you may locate relevant information. Please don’t hesitate to reach out via email or in office hours if you have any questions.
Part 1
What is a reporter gene?
Cite two sources for additional background information
Review Chapter 1, section 1.7 for a reminder of the scientific process and some characteristics of reliable sources of information
Part 2
What impact would changing a promoter have on gene expression?
Chapter 11, starting at 11.1
Develop a hypothesis related to this particular scenario
Chapter 1, sections 1.4 and 1.5 review the characteristics of a hypothesis and how they can be tested experimentally
Develop a rough procedure that uses the listed supplies/reagents to test your hypothesis
You will use information from 12.2 in your book, the lab simulations, and the videos above to generate the necessary plasmids and conduct the transformation.
Part 3
Generate mock results
www.random.org
Part 4
Draw your chart as directed based on the mock results
Part 5
Discuss/interpret your results and plan next steps